Neuronal transcriptional repressor REST suppresses an Atoh7-independent program for initiating retinal ganglion cell development.
Dev Biol
; 349(1): 90-9, 2011 Jan 01.
Article
en En
| MEDLINE
| ID: mdl-20969844
ABSTRACT
As neuronal progenitors differentiate into neurons, they acquire a unique set of transcription factors. The transcriptional repressor REST prevents progenitors from undergoing differentiation. Notably, REST binding sites are often associated with retinal ganglion cell (RGC) genes whose expression in the retina is positively controlled by Atoh7, a factor essential for RGC formation. The key regulators that enable a retinal progenitor cell (RPC) to commit to an RGC fate have not been identified. We show here that REST suppresses RGC gene expression in RPCs. REST inactivation causes aberrant expression of RGC transcription factors in proliferating RPCs, independent of Atoh7, resulting in increased RGC formation. Strikingly, inactivating REST in Atoh7-null retinas restores transcription factor expression, which partially activates downstream RGC genes but is insufficient to prevent RGC loss. Our results demonstrate an Atoh7-independent program for initial activation of RGC genes and suggest a novel role for REST in preventing premature expression in RPCs.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Proteínas Represoras
/
Células Ganglionares de la Retina
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Diferenciación Celular
/
Regulación del Desarrollo de la Expresión Génica
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Factores de Transcripción con Motivo Hélice-Asa-Hélice Básico
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Proteínas del Tejido Nervioso
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Dev Biol
Año:
2011
Tipo del documento:
Article
País de afiliación:
Estados Unidos