Let-7b-mediated suppression of basigin expression and metastasis in mouse melanoma cells.
Exp Cell Res
; 317(4): 445-51, 2011 Feb 15.
Article
en En
| MEDLINE
| ID: mdl-21087605
ABSTRACT
Basigin (Bsg), also called extracellular matrix metalloproteinase inducer (EMMPRIN), is highly expressed on the surface of tumor cells and stimulates adjacent fibroblasts or tumor cells to produce matrix metalloproteinases (mmps). It has been shown that Bsg plays an important role in growth, development, cell differentiation, and tumor progression. MicroRNAs (miRNAs) are a class of short endogenous non-protein coding RNAs of 20-25 nucleotides (nt) that function as post-transcriptional regulators of gene expression by base-pairing to their target mRNAs and thereby mediate cleavage of target mRNAs or translational repression. In this study, let-7b, one of the let-7 family members, was investigated for its effect on the growth and invasiveness of the mouse melanoma cell line B16-F10. We have shown that let-7b can suppress the expression of Bsg in B16-F10 cells and also provided evidence that this suppression could result in the indirect suppression of mmp-9. The ability of B16-F10 cells transfected with let-7b to invade or migrate was significantly reduced. In addition, let-7b transfected B16-F10 cells displayed an inhibition of both cellular proliferation and colony formation. Furthermore, it was shown that the overexpression of let-7b in B16-F10 cells could reduce lung metastasis. Taken together, the present study identifies let-7b as a tumor suppressor that represses cancer cell proliferation and migration as well as tumor metastasis in mouse melanoma cells.
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1
Bases de datos:
MEDLINE
Asunto principal:
MicroARNs
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Basigina
/
Melanoma
/
Metástasis de la Neoplasia
Límite:
Animals
Idioma:
En
Revista:
Exp Cell Res
Año:
2011
Tipo del documento:
Article
País de afiliación:
Taiwán