Global regulation on microRNA in hepatitis B virus-associated hepatocellular carcinoma.
OMICS
; 15(3): 187-91, 2011 Mar.
Article
en En
| MEDLINE
| ID: mdl-21319996
ABSTRACT
Recent work has revealed the causative links between deregulation of microRNAs (miRNAs) and cancer development. In hepatocellular carcinoma (HCC), aberrant expression of miRNAs has been observed, but the molecular mechanisms that contribute to such changes remains to be elucidated. Here, we reported the analysis of miRNA expression in 94 pairs of tumor and adjacent nontumor tissues from HBV-associated HCC in Chinese patients. We found miRNAs were aberrantly expressed in HCC tissues. To investigate the cause of such deregulation, we detected changes in DNA copy number by measuring locus-specific hybridization intensity, and found changes in expression of several miRNAs are correlated with genomic amplification or deletion. For example, the genomic regions of miR-30d and miR-151 were amplified in â¼50% of HCC tumor tissues, and the expressions of these miRNAs are significantly correlated with DNA copy number. We also employed cDNA microarray data, and provide evidence that key regulators of the miRNA biosynthetic pathway, including DROSHA, DGCR8, AGO1, and AGO2, are frequently overexpressed in HCC. This study provides molecular clues that may contribute to the global changes of miRNA expression in HCC.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Virus de la Hepatitis B
/
Carcinoma Hepatocelular
/
MicroARNs
/
Neoplasias Hepáticas
Tipo de estudio:
Risk_factors_studies
Límite:
Humans
Idioma:
En
Revista:
OMICS
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2011
Tipo del documento:
Article
País de afiliación:
Singapur