RNA 3' processing functions of Arabidopsis FCA and FPA limit intergenic transcription.
Proc Natl Acad Sci U S A
; 108(20): 8508-13, 2011 May 17.
Article
en En
| MEDLINE
| ID: mdl-21536901
ABSTRACT
The RNA-binding proteins FCA and FPA were identified based on their repression of the flowering time regulator FLC but have since been shown to have widespread roles in the Arabidopsis thaliana genome. Here, we use whole-genome tiling arrays to show that a wide spectrum of genes and transposable elements are misexpressed in the fca-9 fpa-7 (fcafpa) double mutant at two stages of seedling development. There was a significant bias for misregulated genomic segments mapping to the 3' region of genes. In addition, the double mutant misexpressed a large number of previously unannotated genomic segments corresponding to intergenic regions. We characterized a subset of these misexpressed unannotated segments and established that they resulted from extensive transcriptional read-through, use of downstream polyadenylation sites, and alternative splicing. In some cases, the transcriptional read-through significantly reduced expression of the associated genes. FCA/FPA-dependent changes in DNA methylation were found at several loci, supporting previous associations of FCA/FPA function with chromatin modifications. Our data suggest that FCA and FPA play important roles in the A. thaliana genome in RNA 3' processing and transcription termination, thus limiting intergenic transcription.
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1
Bases de datos:
MEDLINE
Asunto principal:
Transcripción Genética
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Proteínas de Unión al ARN
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Genes de Plantas
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Arabidopsis
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Genoma de Planta
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Proteínas de Arabidopsis
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Procesamiento de Término de ARN 3'
Idioma:
En
Revista:
Proc Natl Acad Sci U S A
Año:
2011
Tipo del documento:
Article
País de afiliación:
Reino Unido