NAADP mediates ATP-induced Ca2+ signals in astrocytes.
FEBS Lett
; 585(14): 2300-6, 2011 Jul 21.
Article
en En
| MEDLINE
| ID: mdl-21664355
ABSTRACT
Intracellular Ca(2+) signals provide astrocytes with a specific form of excitability that enables them to regulate synaptic transmission. In this study, we demonstrate that NAADP-AM, a membrane-permeant analogue of the new second messenger nicotinic acid-adenine dinucleotide phosphate (NAADP), mobilizes Ca(2+) in astrocytes and that the response is blocked by Ned-19, an antagonist of NAADP signalling. We also show that NAADP receptors are expressed in lysosome-related acidic vesicles. Pharmacological disruption of either NAADP or lysosomal signalling reduced Ca(2+) responses induced by ATP and endothelin-1, but not by bradykinin. Furthermore, ATP increased endogenous NAADP levels. Overall, our data provide evidence for NAADP being an intracellular messenger for agonist-mediated calcium signalling in astrocytes.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Adenosina Trifosfato
/
Astrocitos
/
Calcio
/
Señalización del Calcio
/
NADP
Límite:
Animals
Idioma:
En
Revista:
FEBS Lett
Año:
2011
Tipo del documento:
Article
País de afiliación:
España