Highly porous electrospun nanofibers enhanced by ultrasonication for improved cellular infiltration.
Tissue Eng Part A
; 17(21-22): 2695-702, 2011 Nov.
Article
en En
| MEDLINE
| ID: mdl-21682540
ABSTRACT
A significant problem that affects tissue-engineered electrospun nanofibrous scaffolds is poor infiltration of cells into the three-dimensional (3D) structure. Physical manipulation can enhance cellular infiltration into electrospun scaffolds. The porosity of electrospun nanofibers was highly enlarged by ultrasonication in an aqueous solution. The porosity and related property changes on a series of nanofibers were observed to be dependent on ultrasonication time and energy. To evaluate cell infiltration into the scaffold, fibroblasts were seeded onto these nanofibers and cultured for different lengths of time. The penetration levels of these cells into the scaffold were monitored using confocal lazer scanning microscopy. The cell infiltration potential was greatly increased with regard to an increase in pore size and porosity. These 3D nanofibrous scaffolds fabricated by an ultrasonication process allowed cells to infiltrate easily into the scaffold. This approach shows great promise for design of cell permeable nanofibrous scaffolds for tissue-engineering applications.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Ingeniería de Tejidos
/
Andamios del Tejido
/
Nanofibras
Idioma:
En
Revista:
Tissue Eng Part A
Asunto de la revista:
BIOTECNOLOGIA
/
HISTOLOGIA
Año:
2011
Tipo del documento:
Article