[Correction of the alkylnitrite method for the measurement of ethanol in blood: direct experimental evidence].
Sud Med Ekspert
; 54(2): 42-5, 2011.
Article
en Ru
| MEDLINE
| ID: mdl-21735717
ABSTRACT
A coefficient (multiplier) for the correction of a systemic inaccuracy of ethanol detection in the blood by the alkylnitrite method arising from calibration against an aqueous solution has been improved. The experiment was carried out as a multilaboratory study (with the participation of six laboratories). The value of the coefficient was estimated at 0.82 +/- 0.014 (SD) instead of 0.95 computed earlier (CV = 1.7%). The main factor influencing the value of the coefficient turned out to be the time of preparation of the model ethanol-blood mixture for comparison with the aqueous solution. The coefficient of 0.82 was obtained using fresh blood samples that of 0.93 to 0.96 with blood samples previously stored during 3-10 days. The results of the study suggest different ethanol distribution patterns in vivo and in vitro in the aqueous phase of the blood stored for a few days.
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Bases de datos:
MEDLINE
Asunto principal:
Manejo de Especímenes
/
Análisis Químico de la Sangre
/
Etanol
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
Ru
Revista:
Sud Med Ekspert
Año:
2011
Tipo del documento:
Article