Comprehensive epigenetic analysis using oral rinse samples: a pilot study.
J Oral Maxillofac Surg
; 70(6): 1486-94, 2012 Jun.
Article
en En
| MEDLINE
| ID: mdl-21821326
ABSTRACT
PURPOSE:
To prove that chromatin immunoprecipitation assay can be performed with oral rinse samples and to develop a protocol for comprehensive analysis of functional interactions among DNA methylation, histone modification, and gene expression using such samples. MATERIALS ANDMETHODS:
Eleven cancer cell lines and oral rinse samples from 10 patients with oral squamous cell carcinoma and 3 healthy subjects were examined. The expression of CDKN2A, a tumor suppressor gene, was determined by reverse transcription/polymerase chain reaction and immunohistochemistry. Promoter DNA methylation was assessed by methylation-specific polymerase chain reaction. Chromatin modifications were analyzed by a chromatin immunoprecipitation assay using antibodies for dimethylation and acetylation of lysine 9 of histone H3.RESULTS:
Epigenetic control of CDK2NA was observed in vitro in 11 cancer cell lines. Using the present protocol, comprehensive epigenetic analysis could be successfully performed with oral rinse samples. All patients were comfortable using the prescribed amount (16 mL) of normal saline to rinse their mouths. Nine patients (90%) and 1 healthy subject (33%) showed dimethylation of lysine 9 of histone H3. Moreover, 8 patients (80%) showed hypoacetylation of lysine 9 of histone H3, which was not observed in healthy subjects.CONCLUSIONS:
The present study showed for the first time that chromatin modifications can be analyzed using oral rinse samples by chromatin immunoprecipitation analysis. To evaluate the contribution of histone modifications for carcinogenesis of oral squamous cell carcinoma, studies including a larger number of subjects should be conducted in the future.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Neoplasias de la Boca
/
Histonas
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Carcinoma de Células Escamosas
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Inhibidor p16 de la Quinasa Dependiente de Ciclina
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Epigénesis Genética
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Inmunoprecipitación de Cromatina
/
Mucosa Bucal
Tipo de estudio:
Observational_studies
/
Risk_factors_studies
Límite:
Adult
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Aged
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Aged80
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Revista:
J Oral Maxillofac Surg
Año:
2012
Tipo del documento:
Article
País de afiliación:
Japón