The application of molecular diagnostic studies interrogating EGFR and KRAS mutations to stained cytologic smears of lung carcinoma.
Am J Clin Pathol
; 136(4): 564-71, 2011 Oct.
Article
en En
| MEDLINE
| ID: mdl-21917678
ABSTRACT
EGFR and KRAS mutation analyses are of increasing importance for guiding the treatment of non-small cell lung carcinomas. Insufficient cellularity of cell blocks can represent an impediment to the performance of these tests. We investigated the usefulness of cytologic direct smears as an alternative specimen source for mutation testing. Tumor cell-enriched areas from freshly prepared and archived rapid Romanowsky-stained direct smears in 33 cases of lung carcinoma were microdissected for DNA isolation and evaluated for EGFR and KRAS mutations. EGFR mutations were detected in 3 adenocarcinomas; 2 tumors had the L858R substitution and 1 an exon 19 deletion. KRAS mutations affecting codon 12, 13, or 61 were detected in 11 cases (8 adenocarcinomas and 3 non-small cell carcinomas). EGFR and KRAS mutations were mutually exclusive. Hence, archived and freshly prepared direct smears represent a robust and valuable specimen source for molecular studies, especially when cell blocks exhibit insufficient cellularity.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
ADN de Neoplasias
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Análisis Mutacional de ADN
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Adenocarcinoma
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Proteínas Proto-Oncogénicas
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Carcinoma de Pulmón de Células no Pequeñas
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Proteínas ras
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Genes erbB-1
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Neoplasias Pulmonares
Tipo de estudio:
Diagnostic_studies
Límite:
Female
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Humans
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Male
Idioma:
En
Revista:
Am J Clin Pathol
Año:
2011
Tipo del documento:
Article
País de afiliación:
Estados Unidos