Rhodanobacter denitrificans sp. nov., isolated from nitrate-rich zones of a contaminated aquifer.
Int J Syst Evol Microbiol
; 62(Pt 10): 2457-2462, 2012 Oct.
Article
en En
| MEDLINE
| ID: mdl-22140175
ABSTRACT
Bacterial strains 2APBS1(T) and 116-2 were isolated from the subsurface of a nuclear legacy waste site where the sediments are co-contaminated with large amounts of acids, nitrate, metal radionuclides and other heavy metals. A combination of physiological and genetic assays indicated that these strains represent the first member of the genus Rhodanobacter shown to be capable of complete denitrification. Cells of strain 2APBS1(T) and 116-2 were Gram-negative, non-spore-forming rods, 3-5 µm long and 0.25-0.5 µm in diameter. The isolates were facultative anaerobes, and had temperature and pH optima for growth of 30 °C and pH 6.5; they were able to tolerate up to 2.0â% NaCl, although growth improved in its absence. Strains 2APBS1(T) and 116-2 contained fatty acid and quinone (ubiquinone-8; 100â%) profiles that are characteristic features of the genus Rhodanobacter. Although strains 2APBS1(T) and 116-2 shared high 16S rRNA gene sequence similarity with Rhodanobacter thiooxydans LCS2(T) (>99â%), levels of DNA-DNA relatedness between these strains were substantially below the 70â% threshold used to designate novel species. Thus, based on genotypic, phylogenetic, chemotaxonomic and physiological differences, strains 2APBS1(T) and 116-2 are considered to represent a single novel species of the genus Rhodanobacter, for which the name Rhodanobacter denitrificans sp. nov. is proposed. The type strain is 2APBS1(T) (â=âDSM 23569(T)â=âJCM 17641(T)).
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Bases de datos:
MEDLINE
Asunto principal:
Filogenia
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Agua Subterránea
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Xanthomonadaceae
Idioma:
En
Revista:
Int J Syst Evol Microbiol
Asunto de la revista:
MICROBIOLOGIA
Año:
2012
Tipo del documento:
Article
País de afiliación:
Estados Unidos