Characterization of glycan binding specificities of influenza B viruses with correlation with hemagglutinin genotypes and clinical features.
J Med Virol
; 84(4): 679-85, 2012 Apr.
Article
en En
| MEDLINE
| ID: mdl-22337309
ABSTRACT
The carbohydrate binding specificities are different among avian and human influenza A viruses and may affect the tissue tropism and transmission of these viruses. The glycan binding biology for influenza B, however, has not been systematically characterized. Glycan binding specificities of influenza B viral isolates were analyzed and correlated to hemagglutinin (HA) genotypes and clinical manifestations. A newly developed solution glycan array was applied to characterize the receptor binding specificities of influenza B virus clinical isolates from 2001 to 2007 in Taiwan. Thirty oligosaccharides which include α-2,3 and α-2,6 linkage glycans were subjected to analysis. The glycan binding patterns of 53 influenza B isolates could be categorized into three groups and were well correlated to their HA genotypes. The Yamagata-like strains predominantly bound to α-2,6-linkage glycan (2429, 83%) while Victoria-like strains preferentially bound to both α-2,3- and α-2,6-linkage glycans (1324, 54%). A third group of viruses bound to sulfated glycans and these all belonged to Victoria-like strains. Based on the HA sequences, Asn-163, Glu-198, Ala-202, and Lys-203 were conserved among Victoria-like strains which may influence their carbohydrate recognition. The viruses bound to dual type glycans were more likely to be associated with the development of bronchopneumonia and gastrointestinal illness than those bound only to α-2,6 sialyl glycans (P < 0.05). Glycan binding analyses provide additional information to monitor the antigenic shift, tissue tropism, and transmission capability of influenza B viruses, and will contribute to virus surveillance and vaccine strain selection.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Virus de la Influenza B
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Polisacáridos
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Glicoproteínas Hemaglutininas del Virus de la Influenza
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Gripe Humana
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Acoplamiento Viral
Límite:
Humans
País/Región como asunto:
Asia
Idioma:
En
Revista:
J Med Virol
Año:
2012
Tipo del documento:
Article
País de afiliación:
Taiwán