Structure of the Fab-labeled "breathing" state of native poliovirus.
J Virol
; 86(10): 5959-62, 2012 May.
Article
en En
| MEDLINE
| ID: mdl-22398295
ABSTRACT
At 37°C, the structure of poliovirus is dynamic, and internal polypeptides VP4 and N terminus of VP1 (residues 1 to 53) externalize reversibly. An Fab fragment of a monospecific antibody, which binds to residues 39 to 55 of VP1, was utilized to locate the N termini of VP1 in native (160S) particles in this "breathing" state. Fab and virus were mixed and imaged via cryogenic electron microscopy. The resulting reconstruction showed the capsid expands similarly to the irreversibly altered cell entry intermediate (135S) particle, but the N terminus of VP1 is located near the 2-fold axes, instead of the "propeller tip" as in 135S particles.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Fragmentos Fab de Inmunoglobulinas
/
Poliovirus
/
Proteínas de la Cápside
Límite:
Humans
Idioma:
En
Revista:
J Virol
Año:
2012
Tipo del documento:
Article
País de afiliación:
Estados Unidos