Recombinant protein expression and purification: a comprehensive review of affinity tags and microbial applications.
Biotechnol J
; 7(5): 620-34, 2012 May.
Article
en En
| MEDLINE
| ID: mdl-22442034
ABSTRACT
Protein fusion tags are indispensible tools used to improve recombinant protein expression yields, enable protein purification, and accelerate the characterization of protein structure and function. Solubility-enhancing tags, genetically engineered epitopes, and recombinant endoproteases have resulted in a versatile array of combinatorial elements that facilitate protein detection and purification in microbial hosts. In this comprehensive review, we evaluate the most frequently used solubility-enhancing and affinity tags. Furthermore, we provide summaries of well-characterized purification strategies that have been used to increase product yields and have widespread application in many areas of biotechnology including drug discovery, therapeutics, and pharmacology. This review serves as an excellent literature reference for those working on protein fusion tags.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Biotecnología
/
Proteínas Recombinantes de Fusión
/
Ingeniería de Proteínas
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Cromatografía de Afinidad
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Biotechnol J
Asunto de la revista:
BIOTECNOLOGIA
Año:
2012
Tipo del documento:
Article
País de afiliación:
Estados Unidos