Lineage tracing reveals Lgr5+ stem cell activity in mouse intestinal adenomas.
Science
; 337(6095): 730-5, 2012 Aug 10.
Article
en En
| MEDLINE
| ID: mdl-22855427
ABSTRACT
The concept that tumors are maintained by dedicated stem cells, the so-called cancer stem cell hypothesis, has attracted great interest but remains controversial. Studying mouse models, we provide direct, functional evidence for the presence of stem cell activity within primary intestinal adenomas, a precursor to intestinal cancer. By "lineage retracing" using the multicolor Cre-reporter R26R-Confetti, we demonstrate that the crypt stem cell marker Lgr5 (leucine-rich repeat-containing heterotrimeric guanine nucleotide-binding protein-coupled receptor 5) also marks a subpopulation of adenoma cells that fuel the growth of established intestinal adenomas. These Lgr5(+) cells, which represent about 5 to 10% of the cells in the adenomas, generate additional Lgr5(+) cells as well as all other adenoma cell types. The Lgr5(+) cells are intermingled with Paneth cells near the adenoma base, a pattern reminiscent of the architecture of the normal crypt niche.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Células Madre Neoplásicas
/
Adenoma
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Receptores Acoplados a Proteínas G
/
Neoplasias Intestinales
Límite:
Animals
Idioma:
En
Revista:
Science
Año:
2012
Tipo del documento:
Article
País de afiliación:
Países Bajos