[Effect of Ras signal regulating Cox7a2 expression and its sub-cellular location in testicular interstitial cell].
Beijing Da Xue Xue Bao Yi Xue Ban
; 44(4): 507-10, 2012 Aug 18.
Article
en Zh
| MEDLINE
| ID: mdl-22898835
ABSTRACT
OBJECTIVE:
To investigate the co-sub-cellular-location of Cox7a2 and Ras.METHODS:
Ras and its mutant plasmid were cloned by RT-PCR and sequence analysis. Cox7a2-pEYFP-N1, Ras-pEYFP-N1 and N17-Ras-pEYFP-N1 fluorescent protein vectors were constructed and transfected into TM3 cells.RESULTS:
Cox7a2 was located in the mitochondria, but its location was changed by the expression of Ras. When the dominant negative ras was expressed in the cells, the Cox7a2 located into the mitochondria again.CONCLUSION:
Cox7a2 mediated testosterone production, which might be at least in part related with the Ras signaling pathway. Ras may be the regulating target and further investigation is needed to make it clear.
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Bases de datos:
MEDLINE
Asunto principal:
Complejo IV de Transporte de Electrones
/
Proteínas ras
/
Células Intersticiales del Testículo
Límite:
Animals
Idioma:
Zh
Revista:
Beijing Da Xue Xue Bao Yi Xue Ban
Asunto de la revista:
MEDICINA
Año:
2012
Tipo del documento:
Article
País de afiliación:
China