Multiscale characterization of a chimeric biomimetic polypeptide for stem cell culture.
Bioinspir Biomim
; 7(4): 046007, 2012 Dec.
Article
en En
| MEDLINE
| ID: mdl-22917962
Mesenchymal stem cells have attracted great interest in the field of tissue engineering and regenerative medicine because of their multipotentiality and relative ease of isolation from adult tissues. The medical application of this cellular system requires the inclusion in a growth and delivery scaffold that is crucial for the clinical effectiveness of the therapy. In particular, the ideal scaffolding material should have the needed porosity and mechanical strength to allow a good integration with the surrounding tissues, but it should also assure high biocompatibility and full resorbability. For such a purpose, protein-inspired biomaterials and, in particular, elastomeric-derived polypeptides are playing a major role, in which they are expected to fulfil many of the biological and mechanical requirements. A specific chimeric protein, designed starting from elastin, resilin and collagen sequences, was characterized over different length scales. Single-molecule mechanics, aggregation properties and compatibility with human mesenchymal stem cells were tested, showing that the engineered compound is a good candidate as a stem cell scaffold to be used in tissue engineering applications.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Péptidos
/
Proteínas de la Matriz Extracelular
/
Andamios del Tejido
/
Células Madre Mesenquimatosas
Límite:
Humans
Idioma:
En
Revista:
Bioinspir Biomim
Asunto de la revista:
BIOLOGIA
/
ENGENHARIA BIOMEDICA
Año:
2012
Tipo del documento:
Article
País de afiliación:
Italia