Near-surface oxidized sulfur modifications and self-assembly of thiol-modified aptamer on Au thin film substrates influenced by piranha treatment.
ACS Appl Mater Interfaces
; 4(11): 5945-8, 2012 Nov.
Article
en En
| MEDLINE
| ID: mdl-23039103
ABSTRACT
Self-assembly of thiol-modified oligonucleotides on Au films has great importance for biosensor applications. Prior to the self-assembly, a piranha treatment (PT) is commonly used to clean the Au surface. Here we report that near-surface oxidized sulfur modifications on Au thin films by PT for longer than 60 s have serious effects on the self-assembled monolayer (SAM) formation of thiol-modified single-stranded thrombin binding aptamer (s-TBA), and a PT time of 10-30 s is optimal for s-TBA SAM formation. These results have important implication to SAM formation of biomolecules, especially for the thiol-modified ones where a careful consideration of this key step could significantly enhance the SAM formation and biosensor performance.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Compuestos de Sulfhidrilo
/
Azufre
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Técnicas Biosensibles
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Oro
/
Membranas Artificiales
Idioma:
En
Revista:
ACS Appl Mater Interfaces
Asunto de la revista:
BIOTECNOLOGIA
/
ENGENHARIA BIOMEDICA
Año:
2012
Tipo del documento:
Article
País de afiliación:
Canadá