Identification of the protein target of myelin-binding ligands by immunohistochemistry and biochemical analyses.
J Histochem Cytochem
; 61(1): 19-30, 2013 Jan.
Article
en En
| MEDLINE
| ID: mdl-23092790
ABSTRACT
The ability to visualize myelin is important in the diagnosis of demyelinating disorders and the detection of myelin-containing nerves during surgery. The development of myelin-selective imaging agents requires that a defined target for these agents be identified and that a robust assay against the target be developed to allow for assessment of structure-activity relationships. We describe an immunohistochemical analysis and a fluorescence polarization binding assay using purified myelin basic protein (MBP) that provides quantitative evidence that MBP is the molecular binding partner of previously described myelin-selective fluorescent dyes such as BMB, GE3082, and GE3111.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Proteína Básica de Mielina
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
En
Revista:
J Histochem Cytochem
Asunto de la revista:
HISTOCITOQUIMICA
Año:
2013
Tipo del documento:
Article
País de afiliación:
Estados Unidos