Dengue virus type 4 in Niterói, Rio de Janeiro: the role of molecular techniques in laboratory diagnosis and entomological surveillance.
Mem Inst Oswaldo Cruz
; 107(7): 940-5, 2012 Nov.
Article
en En
| MEDLINE
| ID: mdl-23147153
ABSTRACT
In Niterói, state of Rio de Janeiro, dengue virus type 4 (DENV-4) was isolated for the first time in March 2011. We analysed the laboratory findings of the first cases and evaluated the use of molecular techniques for the detection of DENV-4 in Aedes aegypti that were field-caught. Conventional reverse transcriptase-polymerase chain reaction (RT-PCR) and Simplexa™ Dengue real-time RT-PCR confirmed DENV-4 infection in all cases. Additionally, DENV-4 was confirmed in a female Ae. aegypti with 1.08 x 10(3) copies/mL of virus, as determined by quantitative real-time RT-PCR. This is the first time the Simplexa™ Dengue real-time assay has been used for the classification of cases of infection and for entomological investigations. The use of these molecular techniques was shown to be important for the surveillance of dengue in humans and vectors.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Aedes
/
Dengue
/
Virus del Dengue
/
Insectos Vectores
Tipo de estudio:
Diagnostic_studies
/
Screening_studies
Límite:
Animals
/
Female
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Humans
/
Male
País/Región como asunto:
America do sul
/
Brasil
Idioma:
En
Revista:
Mem Inst Oswaldo Cruz
Año:
2012
Tipo del documento:
Article
País de afiliación:
Brasil