Recombinant single-chain antibody with the Trojan peptide penetratin positioned in the linker region enables cargo transfer across the blood-brain barrier.
Appl Biochem Biotechnol
; 169(1): 159-69, 2013 Jan.
Article
en En
| MEDLINE
| ID: mdl-23160949
ABSTRACT
Delivery of therapeutic proteins into tissues and across the blood-brain barrier (BBB) is limited by the size and biochemical properties of the proteins. Efficient delivery across BBB is generally restricted to small, highly lipophilic molecules. However, in the last decades, several peptides that can pass cell membranes have been identified. It has been shown that these peptides are also capable of delivering large hydrophilic cargoes into cells and are therefore a powerful biological tool for transporting drugs across cell membranes and even into the brain. We designed and prepared a single-chain antibody fragment (scFvs), specific for the pathological form of the prion protein (PrP(Sc)), where a cell-penetrating peptide (CPP) was used as a linker between the two variable domains of the scFv. The intravenously administered recombinant scFv-CPP was successfully targeted to and delivered into mouse brain cells. Our single-chain antibody fragments are of special interest in view of possible therapeutic reagents design not only for prion diseases but also for other neurodegenerative diseases.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Barrera Hematoencefálica
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Sistemas de Liberación de Medicamentos
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Anticuerpos de Cadena Única
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Péptidos de Penetración Celular
Tipo de estudio:
Evaluation_studies
/
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Appl Biochem Biotechnol
Año:
2013
Tipo del documento:
Article
País de afiliación:
Eslovenia