Apicularen A acetate induces cell death via AIF translocation and disrupts the microtubule network by down-regulating tubulin in HM7 human colon cancer cells.
Biochem Biophys Res Commun
; 434(3): 634-40, 2013 May 10.
Article
en En
| MEDLINE
| ID: mdl-23583412
ABSTRACT
Apicularen A is a novel antitumor agent and strongly induces death in tumor cells. In this study, we synthesized apicularen A acetate, an acetyl derivative of apicularen A, and investigated its antitumor effect and mechanism in HM7 colon cancer cells. Apicularen A acetate induced apoptotic cell death and caspase-3 activation; however, the pan-caspase inhibitor Z-VAD-fmk could not prevent this cell death. Apicularen A acetate induced the loss of mitochondrial membrane potential and the translocation of apoptosis-inducing factor (AIF) from mitochondria. In addition, apicularen A acetate significantly decreased tubulin mRNA and protein levels and induced disruption of microtubule networks. Taken together, these results indicate that the mechanism of apicularen A acetate involves caspase-independent apoptotic cell death and disruption of microtubule architecture.
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1
Bases de datos:
MEDLINE
Asunto principal:
Tubulina (Proteína)
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Regulación hacia Abajo
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Apoptosis
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Neoplasias del Colon
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Compuestos Bicíclicos Heterocíclicos con Puentes
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Factor Inductor de la Apoptosis
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Microtúbulos
Límite:
Humans
Idioma:
En
Revista:
Biochem Biophys Res Commun
Año:
2013
Tipo del documento:
Article