Basic protocols to investigate hMSC behavior onto electrospun fibers.
Methods Mol Biol
; 1058: 109-17, 2013.
Article
en En
| MEDLINE
| ID: mdl-23700278
ABSTRACT
Human mesenchymal stem cells (hMSC) currently represent a major cell resource in the research laboratory, to study differentiated-cell behavior in 3D scaffolds during the regeneration processes. Adhesion and differentiation of stem cells to a specific phenotype are achieved by culturing them in apposite culture media under precise conditions. Meanwhile, hydrolytic degradation of polymeric scaffolds allows implanted cells to synthesize their own extracellular matrix in situ after implantation so that the degeneration of the foreign scaffold is temporally matched by creation of the new innate one. In this context, structural properties and biochemical signals may concur to influence the cell response to the environmental stimuli during the culture. So, it becomes mandatory to introduce robust protocols to treat hMSC alone-before the culture-and in combination with the scaffolds for the next investigation by scanning electron microscopy. Here, we describe the protocols used to manage hMSC before and during the culture in order to obtain more detailed information on cell mechanisms mediated by polymeric scaffolds.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Técnicas de Cultivo de Célula
/
Nanofibras
/
Células Madre Mesenquimatosas
Límite:
Humans
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2013
Tipo del documento:
Article
País de afiliación:
Italia