Structure of an A-form RNA duplex obtained by degradation of 6S RNA in a crystallization droplet.
Acta Crystallogr Sect F Struct Biol Cryst Commun
; 69(Pt 6): 634-9, 2013 Jun.
Article
en En
| MEDLINE
| ID: mdl-23722840
ABSTRACT
In the course of a crystallographic study of a 132 nt variant of Aquifex aeolicus 6S RNA, a crystal structure of an A-form RNA duplex containing 12 base pairs was solved at a resolution of 2.6 Å. In fact, the RNA duplex is part of the 6S RNA and was obtained by accidental but precise degradation of the 6S RNA in a crystallization droplet. 6S RNA degradation was confirmed by microscopic observation of crystals and gel electrophoresis of crystallization droplets. The RNA oligomers obtained form regular A-form duplexes containing three GoU wobble-type base pairs, one of which engages in intermolecular contacts through a ribose-zipper motif at the crystal-packing interface.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
ARN Bacteriano
/
Proteolisis
Idioma:
En
Revista:
Acta Crystallogr Sect F Struct Biol Cryst Commun
Año:
2013
Tipo del documento:
Article
País de afiliación:
Japón