Treatment of ovariectomized rats with 17ß-estradiol increases hepatic delta-6 desaturase enzyme expression and docosahexaenoic acid levels in hepatic and plasma phospholipids.
Prostaglandins Leukot Essent Fatty Acids
; 89(2-3): 81-8, 2013 Aug.
Article
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| MEDLINE
| ID: mdl-23764042
ABSTRACT
Higher docosahexaenoic acid (DHA) in females compared with males suggests ovarian hormones increase DHA production. Eight-week old rats were ovariectomized or sham operated (SHAM), and ovariectomized rats were treated with implanted pellets providing 17ß-estradiol (OVX+E), progesterone (OVX+P), both (OVX+PE) or neither (OVX) for 14 days. Immunoblot and fatty acid analysis were performed on all samples, and microarray analysis was performed on OVX and SHAM groups. Increased Δ6-desaturase in OVX relative to SHAM was observed by microarray (12% higher) and immunoblot (31% higher). OVX+E and OVX+PE rats had 39% and 42% higher Δ6-desaturase content, respectively, compared with OVX. OVX+E and OVX+PE also increased phospholipid DHA concentrations in liver (increase of 34% and 40%, respectively) and plasma (increase of 70% and 74%, respectively) relative to OVX. Progesterone exerted no effect on Δ6-desaturase or DHA. These results indicate that 17ß-estradiol increases DHA through increased Δ6-desaturase, possibly explaining sex differences in DHA.
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Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Fosfolípidos
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Ácidos Docosahexaenoicos
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Estradiol
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Linoleoil-CoA Desaturasa
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Hígado
Límite:
Animals
Idioma:
En
Revista:
Prostaglandins Leukot Essent Fatty Acids
Asunto de la revista:
ENDOCRINOLOGIA
Año:
2013
Tipo del documento:
Article