Comparison of different IRT-PAP protocols to screen newborns for cystic fibrosis in three central European populations.
J Cyst Fibros
; 13(1): 15-23, 2014 Jan.
Article
en En
| MEDLINE
| ID: mdl-23891278
ABSTRACT
BACKGROUND:
In recent years different IRT/PAP protocols have been evaluated, but the individual performance remains unclear. To optimize the IRT/PAP strategy we compared protocols from three regional CF newborn screening centers (Heidelberg, Dresden, and Prague).METHODS:
We evaluated the effect of elevating the IRT-cut-off from 50 to 65 µg/l (~97.5th to ~99.0th percentile), the need of a failsafe protocol (FS, IRT ≥ 99.9th percentile) and the relative performance using either two IRT-dependent PAP-cut-offs or one PAP-cut-off.FINDINGS:
Elevation of the IRT cut-off to 65 µg/l (~99.0th percentile) increased the PPV significantly (Dresden 0.065 vs. 0.080, p < 0.0001, Prague 0.052 vs. 0.074, p < 0.0001) without reducing sensitivity. All three IRT/PAP protocols showed a trend towards a higher sensitivity with FS than without and when using one PAP-cut-off instead of two IRT-dependent PAP-cut-offs.CONCLUSIONS:
For best performance we suggest an IRT/PAP protocol with an IRT-cut-off close to the 99.0th percentile, FS, and a single PAP-cut-off.Palabras clave
Biochemical screening; CF; CFNBS; Cystic fibrosis; DBS; FS; IRT; Immunoreactive trypsinogen; MI; NBS; Newborn screening; PAP; PI; PS; Pancreatitis associated protein; cystic fibrosis; cystic fibrosis newborn screening; dried blot spot; failsafe strategy; immunoreactive trypsinogen; meconium ileus; newborn screening; pancreatic insufficient; pancreatic sufficient; pancreatitis associated protein
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Tripsinógeno
/
Biomarcadores de Tumor
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Tamizaje Neonatal
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Fibrosis Quística
/
Lectinas Tipo C
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Pruebas con Sangre Seca
/
Antígenos de Neoplasias
Tipo de estudio:
Diagnostic_studies
/
Observational_studies
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Prognostic_studies
/
Risk_factors_studies
Límite:
Humans
/
Newborn
País/Región como asunto:
Europa
Idioma:
En
Revista:
J Cyst Fibros
Año:
2014
Tipo del documento:
Article