Efficient detection of hydrogen bonds in dynamic regions of RNA by sensitivity-optimized NMR pulse sequences.

Dallmann, Andre; Simon, Bernd; Duszczyk, Malgorzata M; Kooshapur, Hamed; Pardi, Arthur; Bermel, Wolfgang; Sattler, Michael

Dallmann, Andre; Simon, Bernd; Duszczyk, Malgorzata M; Kooshapur, Hamed; Pardi, Arthur; Bermel, Wolfgang; Sattler, Michael.

Afiliación

Dallmann A; Institute of Structural Biology, Helmholtz Zentrum München, Ingolstädter Landstr. 1, 85764 Neuherberg (Germany) http://www.nmr.ch.tum.de/; Center for Integrated Protein Science Munich and Chair of Biomolecular NMR, TU München, Lichtenbergstr. 4, 85747 Garching (Germany).

Angew Chem Int Ed Engl ; 52(40): 10487-90, 2013 Sep 27.

Article en En | MEDLINE | ID: mdl-23946052

RESUMEN

Improved Sensitivity: Efficient NMR experiments are presented for determining the secondary structure in large and dynamic RNAs using J-couplings across hydrogen bonds. The experiments provide up to eight-fold improved sensitivity and thus enable detection of base pairs in dynamic regions even in large RNAs.

Asunto(s)

Resonancia Magnética Nuclear Biomolecular/métodos; ARN/química; Secuencia de Bases; Enlace de Hidrógeno; Datos de Secuencia Molecular; Peso Molecular; Conformación de Ácido Nucleico

Palabras clave

NMR spectroscopy; RNA dynamics; RNA secondary structure; base pairs; hydrogen bonds

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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: ARN / Resonancia Magnética Nuclear Biomolecular Tipo de estudio: Diagnostic_studies Idioma: En Revista: Angew Chem Int Ed Engl Año: 2013 Tipo del documento: Article

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