Discrepancies among three laboratory methods for Clostridium difficile detection and a proposal for their optimal use.
FEMS Microbiol Lett
; 350(2): 133-7, 2014 Jan.
Article
en En
| MEDLINE
| ID: mdl-24236508
ABSTRACT
Clostridium difficile is the major cause of nosocomial diarrhoea. Several detection methods are available for the laboratory diagnosis of C. difficile, but these vary in terms of sensitivity and specificity. In this study, we compared the performance of three following laboratory tests to detect C. difficile in-house real-time PCR aiming for toxin B gene (tcdB), EIA for detection of toxins A and B (Premier Toxins A & B) and C. difficile culture in selective medium (bioMerieux). Our results were grouped into three categories as follows (1) C. difficile-associated diarrhoea (CDAD); (2) asymptomatic carriers; and (3) negative results. Among the 113 patients included in the study, 9 (8.0%) were classified as CDAD, 19 (16.8%) were asymptomatic carriers, 76 (67.2%) had negative results and 9 (8.0%) could not be categorized (positive test for C. difficile toxins only). PCR was found to be the most sensitive diagnostic test in our study, with the potential to be used as a screening method for C. difficile colonization/CDAD. Diagnosis of CDAD would be better performed by a combination of PCR and EIA tests.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Enterocolitis Seudomembranosa
/
Clostridioides difficile
/
Técnicas de Tipificación Bacteriana
Tipo de estudio:
Diagnostic_studies
/
Observational_studies
/
Risk_factors_studies
Límite:
Adult
/
Humans
País/Región como asunto:
America do sul
/
Brasil
Idioma:
En
Revista:
FEMS Microbiol Lett
Año:
2014
Tipo del documento:
Article
País de afiliación:
Brasil