Genetic variation in alkaloid accumulation in leaves of Nicotiana.
J Zhejiang Univ Sci B
; 14(12): 1100-9, 2013 Dec.
Article
en En
| MEDLINE
| ID: mdl-24302710
ABSTRACT
Alkaloids are plant secondary metabolites that are widely distributed in Nicotiana species and contribute greatly to the quality of tobacco leaves. Some alkaloids, such as nornicotine and myosmine, have adverse effects on human health. To reduce the content of harmful alkaloids in tobacco leaves through conventional breeding, a genetic study of the alkaloid variation among different genotypes is required. In this study, alkaloid profiles in leaves of five Nicotiana tabacum cultivars and Nicotiana tomentosiformis were investigated. Six alkaloids were identified from all six genotypes via gas chromatograph-mass spectrometry (GC-MS). Significant differences in alkaloid content were observed both among different leaf positions and among cultivars. The contents of nornicotine and myosmine were positively and significantly correlated (R(2)=0.881), and were also separated from those of other alkaloids by clustering. Thus, the genotype plays a major role in alkaloid accumulation, indicating a high potential for manipulation of alkaloid content through traditional breeding.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Nicotiana
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Variación Genética
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Hojas de la Planta
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Alcaloides
Idioma:
En
Revista:
J Zhejiang Univ Sci B
Asunto de la revista:
BIOLOGIA
/
MEDICINA
Año:
2013
Tipo del documento:
Article
País de afiliación:
China