Acridine orange leukocyte fluorography in mice.
Exp Eye Res
; 120: 15-9, 2014 Mar.
Article
en En
| MEDLINE
| ID: mdl-24333760
ABSTRACT
Simultaneous non-invasive visualization of blood vessels and nerves in patients can be obtained in the eye. The retinal vasculature is a target of many retinopathies. Inflammation, readily manifest by leukocyte adhesion to the endothelial lining, is a key pathophysiological mechanism of many retinopathies, making it a valuable and ubiquitous target for disease research. Leukocyte fluorography has been extensively used in the past twenty years; however, fluorescent markers, visualization techniques, and recording methods have differed between studies. The lack of detailed protocol papers regarding leukocyte fluorography, coupled with lack of uniformity between studies, has led to a paucity of standards for leukocyte transit (velocity, adherence, extravasation) in the retina. Here, we give a detailed description of a convenient method using acridine orange (AO) and a commercially available scanning laser ophthalmoscope (SLO, HRA-OCT Spectralis) to view leukocyte behavior in the mouse retina. Normal mice are compared to mice with acute and chronic inflammation. This method can be readily adopted in many research labs.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Arteria Retiniana
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Vena Retiniana
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Naranja de Acridina
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Angiografía con Fluoresceína
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Colorantes Fluorescentes
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Leucocitos
Límite:
Animals
Idioma:
En
Revista:
Exp Eye Res
Año:
2014
Tipo del documento:
Article