Dexamethasone exacerbates cerebral edema and brain injury following lithium-pilocarpine induced status epilepticus.
Neurobiol Dis
; 63: 229-36, 2014 Mar.
Article
en En
| MEDLINE
| ID: mdl-24333865
ABSTRACT
Anti-inflammatory therapies are the current most plausible drug candidates for anti-epileptogenesis and neuroprotection following prolonged seizures. Given that vasogenic edema is widely considered to be detrimental for outcome following status epilepticus, the anti-inflammatory agent dexamethasone is sometimes used in clinic for alleviating cerebral edema. In this study we perform longitudinal magnetic resonance imaging in order to assess the contribution of dexamethasone on cerebral edema and subsequent neuroprotection following status epilepticus. Lithium-pilocarpine was used to induce status epilepticus in rats. Following status epilepticus, rats were either post-treated with saline or with dexamethasone sodium phosphate (10mg/kg or 2mg/kg). Brain edema was assessed by means of magnetic resonance imaging (T2 relaxometry) and hippocampal volumetry was used as a marker of neuronal injury. T2 relaxometry was performed prior to, 48 h and 96 h following status epilepticus. Volume measurements were performed between 18 and 21 days after status epilepticus. Unexpectedly, cerebral edema was worse in rats that were treated with dexamethasone compared to controls. Furthermore, dexamethasone treated rats had lower hippocampal volumes compared to controls 3 weeks after the initial insult. The T2 measurements at 2 days and 4 days in the hippocampus correlated with hippocampal volumes at 3 weeks. Finally, the mortality rate in the first week following status epilepticus increased from 14% in untreated rats to 33% and 46% in rats treated with 2mg/kg and 10mg/kg dexamethasone respectively. These findings suggest that dexamethasone can exacerbate the acute cerebral edema and brain injury associated with status epilepticus.
Palabras clave
BBB; Biomarker; COX-2; CSE; Corticosteroids; DEX; Epilepsy; FOV; Inflammation; IκB; MRI; NSAIDs; ROIs; SE; T(2); TE; TEeff; TR; bloodbrain barrier; convulsive status epilepticus; cyclooxygenase-2; dexamethasone; echo time; echo-train length; effective echo time; etl; fast spin-echo; field of view; fse; inhibitor of kappa-B; magnetic resonance imaging; non-steroidal anti-inflammatory drugs; rHCV; regions of interest; relative hippocampal volume; repetition time; status epilepticus; transverse magnetisation relaxation time constant
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Estado Epiléptico
/
Edema Encefálico
/
Lesiones Encefálicas
/
Dexametasona
/
Corteza Cerebral
/
Antiinflamatorios
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Neurobiol Dis
Asunto de la revista:
NEUROLOGIA
Año:
2014
Tipo del documento:
Article