Comparative assessment of automated nucleic acid sample extraction equipment for biothreat agents.

Kalina, Warren Vincent; Douglas, Christina Elizabeth; Coyne, Susan Rajnik; Minogue, Timothy Devin

Kalina, Warren Vincent; Douglas, Christina Elizabeth; Coyne, Susan Rajnik; Minogue, Timothy Devin.

Afiliación

Kalina WV; Diagnostic Systems Division, United States Army Medical Research Institute of Infectious Disease, Fort Detrick, Maryland, USA.

J Clin Microbiol ; 52(4): 1232-4, 2014 Apr.

Article en En | MEDLINE | ID: mdl-24452173

ABSTRACT

ABSTRACT

Magnetic beads offer superior impurity removal and nucleic acid selection over older extraction methods. The performances of nucleic acid extraction of biothreat agents in blood or buffer by easyMAG, MagNA Pure, EZ1 Advanced XL, and Nordiag Arrow were evaluated. All instruments showed excellent performance in blood; however, the easyMAG had the best precision and versatility.

Asunto(s)

Automatización de Laboratorios/métodos; Armas Biológicas; Técnicas Microbiológicas/métodos; Ácidos Nucleicos/sangre; Ácidos Nucleicos/aislamiento & purificación; Humanos; Técnicas Microbiológicas/instrumentación

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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Ácidos Nucleicos / Técnicas Microbiológicas / Armas Biológicas / Automatización de Laboratorios Límite: Humans Idioma: En Revista: J Clin Microbiol Año: 2014 Tipo del documento: Article País de afiliación: Estados Unidos

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