Trastuzumab quantification in serum: a new, rapid, robust ELISA assay based on a mimetic peptide that specifically recognizes trastuzumab.
Anal Bioanal Chem
; 406(18): 4557-61, 2014 Jul.
Article
en En
| MEDLINE
| ID: mdl-24828979
ABSTRACT
Trastuzumab, a humanized monoclonal antibody directed against the epidermal growth factor receptor 2 (HER2), is a milestone in the treatment of HER2-overexpressing breast cancer patients. An enzyme-linked immunosorbent assay (ELISA) for trastuzumab has been developed for routine use in the laboratory to support clinical and pharmacokinetic studies to optimize therapy. The method relies on an antigen peptide linked to a 96-well plate via the streptavidin/biotin system. The peptide sequence mimics the extracellular portion of the HER2 receptor that is recognized by trastuzumab. The calibration range of the assay is 10 to 360 ng/mL per well, corresponding to a trastuzumab serum concentration from 5 to 180 µg/mL with a lower limit of quantification of 10 µg/mL. Validation results demonstrate that trastuzumab can be accurately and precisely quantified in human serum using this assay. The procedure was also tested in sera obtained from breast cancer patients to evaluate trastuzumab serum levels, confirming the applicability of method that could be a valid assay to use in daily laboratory practice.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Péptidos
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Neoplasias de la Mama
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Ensayo de Inmunoadsorción Enzimática
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Anticuerpos Monoclonales Humanizados
Tipo de estudio:
Clinical_trials
Límite:
Female
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Humans
Idioma:
En
Revista:
Anal Bioanal Chem
Año:
2014
Tipo del documento:
Article
País de afiliación:
Italia