Anterior pituitary autoantibodies in patients with type 1 diabetes mellitus: methodological problems and clinical correlations.
J Endocrinol Invest
; 37(10): 973-8, 2014 Oct.
Article
en En
| MEDLINE
| ID: mdl-25070044
ABSTRACT
BACKGROUND:
Anti-pituitary antibodies (APA) were described in patients with Type 1 Diabetes (T1D) but their prevalence and relevance remain controversial. MATERIALS ANDMETHODS:
We evaluated the APA prevalence in Sardinian sera from 100 T1D patients, 70 Type 2 Diabetes (T2D) patients and 62 healthy controls, using indirect immunofluorescence on bovine pituitary sections. To compare two different substrates, we tested using bovine sections, further T1D patient sera (n = 11, from Pisa) previously analysed for APA on monkey sections, while some T1D Sardinian patient sera (n = 22) were tested on monkey sections. According to preliminary experiments, positivity were considered ≥1200 and ≥120 for bovine and monkey substrates, respectively. RESULTS ANDDISCUSSION:
Using bovine sections, APA were detected in 7/100 Sardinian T1D patients (at 1200 titer) and in none of the other Sardinian sera tested. When the T1D sera from Pisa were tested on bovine and the T1D Sardinian sera were tested on monkey, none of these sera showed corresponding positivity for APA. Pituitary hormone dysfunctions were not found in the 7 APA-positive Sardinian T1D patients. The present study shows that the presence of APA at low-titer is highly related to T1D but not associated with any pituitary dysfunction while the animal species used as substrate appears crucial.CONCLUSION:
Further studies are needed to ascertain whether APA detected by different animal species may have different pathological relevance in T1D and/or whether APA in the long run may predict future anterior pituitary dysfunction.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Hipófisis
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Autoanticuerpos
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Diabetes Mellitus Tipo 1
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Diabetes Mellitus Tipo 2
Tipo de estudio:
Risk_factors_studies
Límite:
Adult
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Animals
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Female
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Humans
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Male
/
Middle aged
País/Región como asunto:
Europa
Idioma:
En
Revista:
J Endocrinol Invest
Año:
2014
Tipo del documento:
Article
País de afiliación:
Italia