Glucocorticoid receptor agonist dexamethasone attenuates renal ischemia/reperfusion injury by up-regulating eNOS/iNOS.
J Huazhong Univ Sci Technolog Med Sci
; 34(4): 516-520, 2014 Aug.
Article
en En
| MEDLINE
| ID: mdl-25135720
ABSTRACT
The aim of this study was to determine the effect of dexamethasone (DEX) on renal ischemia/reperfusion injury (IRI). C57BL/6 mice were randomly divided into Sham group, IRI group and DEX group. The mice in IRI and DEX groups subjected to renal ischemia for 60 min, were treated with saline or DEX (4 mg/kg, i.p.) 60 min prior to I/R. After 24 h of reperfusion, the renal function, renal pathological changes, activation of extracellular signal-regulated kinase (ERK) and glucocorticoid receptor (GR), and the levels of iNOS and eNOS were detected. The results showed DEX significantly decreased the damage to renal function and pathological changes after renal IRI. Pre-treatment with DEX reduced ERK activation and down-regulated the level of iNOS, whereas up-regulated the level of eNOS after renal IRI. DEX could further promote the activation of GR. These findings indicated GR activation confers preconditioning-like protection against acute IRI partially by up-regulating the ratio of eNOS/iNOS.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Dexametasona
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Daño por Reperfusión
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Receptores de Glucocorticoides
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Regulación Enzimológica de la Expresión Génica
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Regulación hacia Arriba
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Óxido Nítrico Sintasa de Tipo II
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Óxido Nítrico Sintasa de Tipo III
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Glucocorticoides
Límite:
Animals
Idioma:
En
Revista:
J Huazhong Univ Sci Technolog Med Sci
Asunto de la revista:
MEDICINA
Año:
2014
Tipo del documento:
Article
País de afiliación:
China