Chromatographic and electrophoretic strategies for the chiral separation and quantification of d- and l-threo methylphenidate in biological matrices.
Biomed Chromatogr
; 28(11): 1554-64, 2014 Nov.
Article
en En
| MEDLINE
| ID: mdl-25196779
ABSTRACT
Commercially available methylphenidate (MPH) exists as a racemic mixture composed of the d- and l-threo enantiomers. Various pharmacokinetic studies of MPH have shown a greater pharmacological potency of the d-threo enantiomer. Furthermore, it was deduced that the stereoselective cleavage of MPH to produce ritalinic acid (RA) by human carboxylesterase results in a higher oral bioavailability of the d-threo enantiomer. As a requirement for pharmaceutical regulation authorities, efforts have been made to determine the differential biological distribution of d- and l-threo MPH and RA enantiomers. In support of these efforts, numerous analytical procedures have been developed for the chiral separation and quantification of MPH enantiomers in a variety of biological matrices. The available methodologies accomplish the enantioseparation and quantification of MPH using gas chromatography, liquid chromatography or capillary electrophoretic techniques coupled with a variety of detectors. The current review discusses the technical procedures involved, and the sensitivity and selectivity of these assays.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Cromatografía Liquida
/
Electroforesis Capilar
/
Cromatografía de Gases y Espectrometría de Masas
/
Metilfenidato
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Biomed Chromatogr
Año:
2014
Tipo del documento:
Article
País de afiliación:
Estados Unidos