Evidence for widespread positive and negative selection in coding and conserved noncoding regions of Capsella grandiflora.
PLoS Genet
; 10(9): e1004622, 2014 Sep.
Article
en En
| MEDLINE
| ID: mdl-25255320
ABSTRACT
The extent that both positive and negative selection vary across different portions of plant genomes remains poorly understood. Here, we sequence whole genomes of 13 Capsella grandiflora individuals and quantify the amount of selection across the genome. Using an estimate of the distribution of fitness effects, we show that selection is strong in coding regions, but weak in most noncoding regions, with the exception of 5' and 3' untranslated regions (UTRs). However, estimates of selection on noncoding regions conserved across the Brassicaceae family show strong signals of selection. Additionally, we see reductions in neutral diversity around functional substitutions in both coding and conserved noncoding regions, indicating recent selective sweeps at these sites. Finally, using expression data from leaf tissue we show that genes that are more highly expressed experience stronger negative selection but comparable levels of positive selection to lowly expressed genes. Overall, we observe widespread positive and negative selection in coding and regulatory regions, but our results also suggest that both positive and negative selection on plant noncoding sequence are considerably rarer than in animal genomes.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Selección Genética
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Sistemas de Lectura Abierta
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Secuencia Conservada
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Regiones no Traducidas
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Capsella
Idioma:
En
Revista:
PLoS Genet
Asunto de la revista:
GENETICA
Año:
2014
Tipo del documento:
Article
País de afiliación:
Canadá