A fruitless upstream region that defines the species specificity in the male-specific muscle patterning in Drosophila.
J Neurogenet
; 29(1): 23-9, 2015 Mar.
Article
en En
| MEDLINE
| ID: mdl-25518733
ABSTRACT
The muscle of Lawrence (MOL) is a male-specific muscle present in the abdomen of some adult Drosophila species. Formation of the MOL depends on innervation by motoneurons that express fruitless, a neural male determinant. Drosophila melanogaster males carry a pair of MOLs in the 5th abdominal segment, whereas D. subobscura males carry a pair in both the 5th and 4th segments. We hypothesized that the fru gene of D. subobscura but not that of D. melanogaster contains a cis element that directs the formation of the additional pair of MOLs. Successively extended 5' DNA fragments to the P1 promoter of D. subobscura or the corresponding fragments that are chimeric (i.e., containing both melanogaster and subobscura elements) were introduced into D. melanogaster and tested for their ability to induce the MOL to locate the hypothetical cis element. We found that a 1.5-2-kb genomic fragment located 4-6-kb upstream of the P1 promoter in D. subobscura but not that of D. melanogaster permits MOL formation in females, provided this fragment is grafted to the distal â¼4-kb segment from D. melanogaster, demonstrating that this genomic fragment of D. subobscura contains a cis element for the MOL induction.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Especificidad de la Especie
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Factores de Transcripción
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Regulación de la Expresión Génica
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Regiones Promotoras Genéticas
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Músculos Abdominales
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Proteínas de Drosophila
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Proteínas del Tejido Nervioso
Límite:
Animals
Idioma:
En
Revista:
J Neurogenet
Año:
2015
Tipo del documento:
Article
País de afiliación:
Japón