Influence of Testosterone on Inflammatory Response in Testicular Cells and Expression of Transcription Factor Foxp3 in T Cells.
Am J Reprod Immunol
; 74(1): 12-25, 2015 Jul.
Article
en En
| MEDLINE
| ID: mdl-25598450
ABSTRACT
PROBLEM:
Previous studies demonstrated a strong association between low androgen levels and reduced capacity to mount an inflammatory response. However, the mechanisms underlying these observations are largely not understood. METHODS OF STUDY Generation of CD4+CD25+Foxp3+ regulatory T cells in Leydig cell-conditioned media was determined by flow cytometry and ELISA. Influence of testosterone on cytokine response was measured in LPS-stimulated testicular macrophages, Sertoli and peritubular cells.RESULTS:
Leydig cell-conditioned media dose-dependently stimulated expression of transcription factor Foxp3 and secretion of IL-10 in splenic CD4+ T cells, an effect abolished by addition of the anti-androgen flutamide. In isolated Sertoli and peritubular cells, testosterone pre-treatment suppressed the LPS-induced inflammatory response on TNF-α mRNA expression, while no effect was evident in testicular macrophages (TM).CONCLUSIONS:
Androgens can influence the immune system under normal conditions by the generation and functional differentiation of regulatory T cells and in testicular inflammation by direct effect on Sertoli and peritubular cells.Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Testosterona
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Interleucina-10
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Linfocitos T Reguladores
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Factores de Transcripción Forkhead
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Células Intersticiales del Testículo
Límite:
Animals
Idioma:
En
Revista:
Am J Reprod Immunol
Año:
2015
Tipo del documento:
Article
País de afiliación:
Alemania