WT1 immunoenzyme staining using SurePath(™) processed urine cytology helps to detect kidney disease.
Cytopathology
; 27(1): 43-9, 2016 Feb.
Article
en En
| MEDLINE
| ID: mdl-25683486
ABSTRACT
OBJECTIVES:
Damage and detachment of podocytes and loss into the urine have been implicated in the progression of kidney diseases. The purpose of this study was to investigate the potential role of urine cytology based on SurePath(™) combined with immunoenzyme staining using Wilms' tumour 1 (WT1) antibody as a podocyte marker in the discrimination of normality and non-renal urinary tract disease from kidney disease.METHODS:
Sixty-six patients with kidney disease, 45 patients with lower urinary tract disease and 30 healthy volunteers were examined. Urine cytology slides were prepared using the SurePath method and immunoenzyme stained with WT1 antibody, and the number of WT1-positive cells was counted.RESULTS:
In kidney disease, WT1-positive cells were found in 33 (50%) of 66 samples. No WT1-positive cells were found in 45 patients with lower urinary tract disease or in 30 healthy volunteers. The positive rates for WT1 varied with disease type, but not significantly immunoglobulin A (IgA) nephropathy, (14/23); membranous glomerulonephritis, (4/10); Henoch-Schönlein purpura nephritis, (3/5); diabetic glomerulopathy, (5/5); minor glomerular abnormality/minimal change nephrotic syndrome (0/4).CONCLUSIONS:
The results suggest that WT1 immunoenzyme staining of urine cytology can be used to detect some types of kidney disease.Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Coloración y Etiquetado
/
Técnicas para Inmunoenzimas
/
Proteínas WT1
/
Podocitos
/
Enfermedades Renales
Tipo de estudio:
Diagnostic_studies
Límite:
Adult
/
Aged
/
Aged80
/
Female
/
Humans
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Male
/
Middle aged
Idioma:
En
Revista:
Cytopathology
Asunto de la revista:
PATOLOGIA
Año:
2016
Tipo del documento:
Article
País de afiliación:
Japón