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A method to quantify FRET stoichiometry with phasor plot analysis and acceptor lifetime ingrowth.
Chen, WeiYue; Avezov, Edward; Schlachter, Simon C; Gielen, Fabrice; Laine, Romain F; Harding, Heather P; Hollfelder, Florian; Ron, David; Kaminski, Clemens F.
Afiliación
  • Chen W; Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, United Kingdom.
  • Avezov E; The Wellcome Trust Medical Research Council Institute of Metabolic Science and National Institute for Health Research, Cambridge Biomedical Research Centre, Cambridge Institute for Medical Research, University of Cambridge, Cambridge, United Kingdom.
  • Schlachter SC; Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, United Kingdom.
  • Gielen F; Department of Biochemistry, University of Cambridge, Cambridge, United Kingdom.
  • Laine RF; Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, United Kingdom.
  • Harding HP; The Wellcome Trust Medical Research Council Institute of Metabolic Science and National Institute for Health Research, Cambridge Biomedical Research Centre, Cambridge Institute for Medical Research, University of Cambridge, Cambridge, United Kingdom.
  • Hollfelder F; Department of Biochemistry, University of Cambridge, Cambridge, United Kingdom.
  • Ron D; The Wellcome Trust Medical Research Council Institute of Metabolic Science and National Institute for Health Research, Cambridge Biomedical Research Centre, Cambridge Institute for Medical Research, University of Cambridge, Cambridge, United Kingdom.
  • Kaminski CF; Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, United Kingdom. Electronic address: cfk23@cam.ac.uk.
Biophys J ; 108(5): 999-1002, 2015 Mar 10.
Article en En | MEDLINE | ID: mdl-25762312
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Transferencia Resonante de Energía de Fluorescencia / Microfluídica Límite: Humans Idioma: En Revista: Biophys J Año: 2015 Tipo del documento: Article País de afiliación: Reino Unido
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Transferencia Resonante de Energía de Fluorescencia / Microfluídica Límite: Humans Idioma: En Revista: Biophys J Año: 2015 Tipo del documento: Article País de afiliación: Reino Unido