Site-specific analysis of protein hydration based on unnatural amino acid fluorescence.
J Am Chem Soc
; 137(15): 4988-92, 2015 Apr 22.
Article
en En
| MEDLINE
| ID: mdl-25815779
ABSTRACT
Hydration of proteins profoundly affects their functions. We describe a simple and general method for site-specific analysis of protein hydration based on the in vivo incorporation of fluorescent unnatural amino acids and their analysis by steady-state fluorescence spectroscopy. Using this method, we investigate the hydration of functionally important regions of dehalogenases. The experimental results are compared to findings from molecular dynamics simulations.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Agua
/
Proteínas
/
Fluorescencia
/
Aminoácidos
Idioma:
En
Revista:
J Am Chem Soc
Año:
2015
Tipo del documento:
Article
País de afiliación:
República Checa