Lectin engineering, a molecular evolutionary approach to expanding the lectin utilities.
Molecules
; 20(5): 7637-56, 2015 Apr 27.
Article
en En
| MEDLINE
| ID: mdl-25923514
ABSTRACT
In the post genomic era, glycomics--the systematic study of all glycan structures of a given cell or organism--has emerged as an indispensable technology in various fields of biology and medicine. Lectins are regarded as "decipherers of glycans", being useful reagents for their structural analysis, and have been widely used in glycomic studies. However, the inconsistent activity and availability associated with the plant-derived lectins that comprise most of the commercially available lectins, and the limit in the range of glycan structures covered, have necessitated the development of innovative tools via engineering of lectins on existing scaffolds. This review will summarize the current state of the art of lectin engineering and highlight recent technological advances in this field. The key issues associated with the strategy of lectin engineering including selection of template lectin, construction of a mutagenesis library, and high-throughput screening methods are discussed.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Polisacáridos
/
Galectinas
/
Lectinas de Plantas
Límite:
Humans
Idioma:
En
Revista:
Molecules
Asunto de la revista:
BIOLOGIA
Año:
2015
Tipo del documento:
Article
País de afiliación:
China