Design and Structure of an Engineered Disulfide-Stabilized Influenza Virus Hemagglutinin Trimer.
J Virol
; 89(14): 7417-20, 2015 Jul.
Article
en En
| MEDLINE
| ID: mdl-25926650
ABSTRACT
We engineered a disulfide-stabilized influenza virus hemagglutinin (HA) trimer, termed HA3-SS, by introducing cysteine residues into the HA stem to covalently bridge the three protomers. HA3-SS has increased thermostability compared to wild-type HA, and binding of head- and stem-targeted antibodies (Abs) is preserved; only minor structural changes are found in the vicinity of the additional disulfide. This platform has been applied to H1 and H3 HAs and provides prospects for design of intact, stabilized influenza virus HA immunogens.
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Bases de datos:
MEDLINE
Asunto principal:
Orthomyxoviridae
/
Glicoproteínas Hemaglutininas del Virus de la Influenza
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Disulfuros
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Anticuerpos Antivirales
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Antígenos Virales
Límite:
Humans
Idioma:
En
Revista:
J Virol
Año:
2015
Tipo del documento:
Article
País de afiliación:
Estados Unidos