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Segregation versus colocalization: orthogonally functionalized binary micropatterned substrates regulate the molecular distribution in focal adhesions.
Guasch, Judith; Conings, Bert; Neubauer, Stefanie; Rechenmacher, Florian; Ende, Karen; Rolli, Claudio G; Kappel, Christian; Schaufler, Viktoria; Micoulet, Alexandre; Kessler, Horst; Boyen, Hans-Gerd; Cavalcanti-Adam, Elisabetta Ada; Spatz, Joachim P.
Afiliación
  • Guasch J; Department of New Materials and Biosystems, Max Planck Institute for Intelligent Systems, Heisenbergstr. 3, Stuttgart, D-70569, Germany.
  • Conings B; Department of Biophysical Chemistry, University of Heidelberg INF 253, Heidelberg, D-69120, Germany.
  • Neubauer S; Institute for Materials Research, Wetenschapspark 1, Diepenbeek, B-3590, Belgium.
  • Rechenmacher F; Department of New Materials and Biosystems, Max Planck Institute for Intelligent Systems, Heisenbergstr. 3, Stuttgart, D-70569, Germany.
  • Ende K; Institute for Advanced Study (IAS) and Center of Integrated Protein Science (CIPSM), Department Chemie, Technische Universität München, Lichtenbergstr. 4, Garching, D-85747, Germany.
  • Rolli CG; Institute for Advanced Study (IAS) and Center of Integrated Protein Science (CIPSM), Department Chemie, Technische Universität München, Lichtenbergstr. 4, Garching, D-85747, Germany.
  • Kappel C; Department of New Materials and Biosystems, Max Planck Institute for Intelligent Systems, Heisenbergstr. 3, Stuttgart, D-70569, Germany.
  • Schaufler V; Department of New Materials and Biosystems, Max Planck Institute for Intelligent Systems, Heisenbergstr. 3, Stuttgart, D-70569, Germany.
  • Micoulet A; Department of Biophysical Chemistry, University of Heidelberg INF 253, Heidelberg, D-69120, Germany.
  • Kessler H; Department of New Materials and Biosystems, Max Planck Institute for Intelligent Systems, Heisenbergstr. 3, Stuttgart, D-70569, Germany.
  • Boyen HG; Department of New Materials and Biosystems, Max Planck Institute for Intelligent Systems, Heisenbergstr. 3, Stuttgart, D-70569, Germany.
  • Cavalcanti-Adam EA; Department of Biophysical Chemistry, University of Heidelberg INF 253, Heidelberg, D-69120, Germany.
  • Spatz JP; Department of New Materials and Biosystems, Max Planck Institute for Intelligent Systems, Heisenbergstr. 3, Stuttgart, D-70569, Germany.
Adv Mater ; 27(25): 3737-47, 2015 Jul 01.
Article en En | MEDLINE | ID: mdl-25981929
ABSTRACT
Orthogonally functionalized binary micropatterned substrates are produced using a novel protocol. The use of adequate peptido-mimetics enables an unprecedented segregation of purified αvß3 and α5ß1 integrins in adjacent microislands and evidences the preference of U2OS cells to colocalize such receptors. Moreover, this tendency can be altered by varying the geometry and composition of the micropatterns.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Movimiento Celular / Adhesiones Focales / Integrina alfa5beta1 / Integrina alfaVbeta3 Límite: Humans Idioma: En Revista: Adv Mater Asunto de la revista: BIOFISICA / QUIMICA Año: 2015 Tipo del documento: Article País de afiliación: Alemania
Texto completo
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Movimiento Celular / Adhesiones Focales / Integrina alfa5beta1 / Integrina alfaVbeta3 Límite: Humans Idioma: En Revista: Adv Mater Asunto de la revista: BIOFISICA / QUIMICA Año: 2015 Tipo del documento: Article País de afiliación: Alemania