[Etiologic diagnosis in meningitis and encephalitis molecular biology techniques]. / Diagnóstico etiológico en meningitis y encefalitis por técnicas de biología molecular.
Rev Chil Pediatr
; 87(1): 24-30, 2016.
Article
en Es
| MEDLINE
| ID: mdl-26493774
ABSTRACT
INTRODUCTION:
The aetiological study of infections of the central nervous system has traditionally been performed using bacterial cultures and, more recently, using polymerase chain reaction (PCR) for herpes simplex virus (HSV). Bacterial cultures may not have good performance, especially in the context of patients who have received antibiotics prior to sampling, and a request for HSV only by PCR reduces the information to only one aetiological agent. The aim of this study is to determine the infectious causes of meningitis and encephalitis, using traditional microbiology and molecular biology to improve the aetiological diagnosis of these diseases. PATIENTS ANDMETHOD:
A prospective study was conducted on 19 patients with suspected meningitis, admitted to the Luis Calvo Mackenna Hospital in Santiago, Chile, from March 1, 2011 to March 30, 2012. After obtaining informed consent, the CSF samples underwent cytochemical study, conventional culture, multiplex PCR for the major producing bacterial meningitis (N. meningitidis, S. pneumoniae, H. influenzae), real-time single PCR for HSV-1 and 2, VZV, EBV, CMV, HHV-6 and enterovirus. Clinical and epidemiological data were also collected from the clinical records.RESULTS:
Of the 19 patients analysed, 2 were diagnosed by conventional methods and 7 by adding molecular biology (increase to 37%). Three patients had meningitis due to S. pneumoniae, one due to Enterobacter cloacae, 2 patients meningoencephalitis HSV-1, and one VZV meningitis.CONCLUSIONS:
The addition of PCR to conventional diagnostic methods in CNS infections increases the probability of finding the causal agent. This allows a more adequate, timely and rational management of the disease.Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Técnicas de Diagnóstico Molecular
/
Encefalitis
/
Meningitis
Tipo de estudio:
Diagnostic_studies
/
Etiology_studies
/
Observational_studies
Límite:
Child, preschool
/
Female
/
Humans
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Infant
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Male
País/Región como asunto:
America do sul
/
Chile
Idioma:
Es
Revista:
Rev Chil Pediatr
Año:
2016
Tipo del documento:
Article