Oral administration of a recombinant attenuated Yersinia pseudotuberculosis strain elicits protective immunity against plague.
Vaccine
; 33(48): 6727-35, 2015 Nov 27.
Article
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| MEDLINE
| ID: mdl-26514425
ABSTRACT
A Yersinia pseudotuberculosis PB1+ (Yptb PB1+) mutant strain combined with chromosome insertion of the caf1R-caf1A-caf1M-caf1 operon and deletions of yopJ and yopK, χ10068 [pYV-ω2 (ΔyopJ315 ΔyopK108) ΔlacZ044caf1R-caf1M-caf1A-caf1] was constructed. Results indicated that gene insertion and deletion did not affect the growth rate of χ10068 compared to wild-type Yptb cultured at 26 °C. In addition, the F1 antigen in χ10068 was synthesized and secreted on the surface of bacteria at 37 °C (mammalian body temperature), not at ambient culture temperature (26 °C). Immunization with χ10068 primed antibody responses and specific T-cell responses to F1 and YpL (Y. pestis whole cell lysate). Oral immunization with a single dose of χ10068 provided 70% protection against a subcutaneous (s.c.) challenge with â¼ 2.6 × 10(5) LD50 of Y. pestis KIM6+ (pCD1Ap) (KIM6+Ap) and 90% protection against an intranasal (i.n.) challenge with â¼ 500 LD50 of KIM6+Ap in mice. Our results suggest that χ10068 can be used as an effective precursor to make a safe vaccine to prevent plague in humans and to eliminate plague circulation among humans and animals.
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Texto completo:
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Bases de datos:
MEDLINE
Asunto principal:
Peste
/
Proteínas Bacterianas
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Yersinia pseudotuberculosis
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Vacuna contra la Peste
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Vaccine
Año:
2015
Tipo del documento:
Article