Phage Conversion for ß-Lactam Antibiotic Resistance of Staphylococcus aureus from Foods.
J Microbiol Biotechnol
; 26(2): 263-9, 2016 Feb.
Article
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| MEDLINE
| ID: mdl-26562692
ABSTRACT
Temperate phages have been suggested to carry virulence factors and other lysogenic conversion genes that play important roles in pathogenicity. In this study, phage TEM123 in wild-type Staphylococcus aureus from food sources was analyzed with respect to its morphology, genome sequence, and antibiotic resistance conversion ability. Phage TEM123 from a mitomycin C-induced lysate of S. aureus was isolated from foods. Morphological analysis under a transmission electron microscope revealed that it belonged to the family Siphoviridae. The genome of phage TEM123 consisted of a double-stranded DNA of 43,786 bp with a G+C content of 34.06%. A bioinformatics analysis of the phage genome identified 43 putative open reading frames (ORFs). ORF1 encoded a protein that was nearly identical to the metallo-ß-lactamase enzymes that degrade ß-lactam antibiotics. After transduction to S. aureus with phage TEM123, the metallo-ß-lactamase gene was confirmed in the transductant by PCR and sequencing analyses. In a ß-lactam antibiotic susceptibility test, the transductant was more highly resistant to ß-lactam antibiotics than S. aureus S133. Phage TEM123 might play a role in the transfer of ß-lactam antibiotic resistance determinants in S. aureus. Therefore, we suggest that the prophage of S. aureus with its exotoxin is a risk factor for food safety in the food chain through lateral gene transfer.
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Bases de datos:
MEDLINE
Asunto principal:
Staphylococcus aureus
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Fagos de Staphylococcus
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Siphoviridae
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Resistencia betalactámica
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Microbiología de Alimentos
Tipo de estudio:
Prognostic_studies
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Risk_factors_studies
Idioma:
En
Revista:
J Microbiol Biotechnol
Año:
2016
Tipo del documento:
Article