Transforming growth factor (TGF) ß1 acted through miR-130b to increase integrin α5 to promote migration of colorectal cancer cells.
Tumour Biol
; 37(8): 10763-73, 2016 Aug.
Article
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| MEDLINE
| ID: mdl-26873488
ABSTRACT
Transforming growth factor (TGF)-ß1 is a significant stimulator of tumor invasion and metastasis. More recently, it has been found that TGF-ß1 acts through microRNAs to regulate their target genes to promote cancer progresses. However, such similar regulation is rarely reported in colorectal cancer (CRC). Here, we observed a decrease in TGF-ß1 expression in CRC specimens, compared with matched adjacent normal tissues. In parallel, there was an increase in miR-130b characterized in the same samples by microarray assay. Further, treatment of CRC cells with TGF-ß1 caused a significant decrease in the expression of miR-130b and an increased CRC cell migration. Luciferase reporter assay revealed that miR-130b directly targeted the 3' untranslated region (3'UTR) region of integrin α5 gene, which encodes a key molecule involved in cell motility. Subsequently, in the overexpression of miR-130b CRC cells, we observed a decreased level of integrin α5 protein. The regulation of integrin α5 by miR-130b was further shown using the miR-130b mimics and inhibitor of miR-130b. And, knockdown miR-130b with inhibitor in the overexpression of miR-130b CRC cells recovered integrin α5 expression and integrin α5-mediated cell motility. Moreover, the inverse relevance between miR-130b and integrin α5 was also observed in CRC specimens. At last, the enhancement of integrin α5 in TGF-ß1-treated cells can be reversed partly when rescuing miR-130b expression. Together, our findings suggested that TGF-ß1 acted through miR-130b to promote integrin α5 expression, resulting in the enhanced migration of CRC cells.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
ARN Neoplásico
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Neoplasias Colorrectales
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Adenocarcinoma
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Regulación Neoplásica de la Expresión Génica
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Integrina alfaV
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MicroARNs
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Factor de Crecimiento Transformador beta1
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Proteínas de Neoplasias
Límite:
Adult
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Aged
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Aged80
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Revista:
Tumour Biol
Asunto de la revista:
NEOPLASIAS
Año:
2016
Tipo del documento:
Article
País de afiliación:
China