Optimization extraction conditions for improving phenolic content and antioxidant activity in Berberis asiatica fruits using response surface methodology (RSM).
Food Chem
; 207: 115-24, 2016 Sep 15.
Article
en En
| MEDLINE
| ID: mdl-27080887
ABSTRACT
This study for the first time designed to optimize the extraction of phenolic compounds and antioxidant potential of Berberis asiatica fruits using response surface methodology (RSM). Solvent selection was done based on the preliminary experiments and a five-factors-three-level, Central Composite Design (CCD). Extraction temperature (X1), sample to solvent ratio (X3) and solvent concentration (X5) significantly affect response variables. The quadratic model well fitted for all the responses. Under optimal extraction conditions, the dried fruit sample mixed with 80% methanol having 3.0 pH in a ratio of 150 and the mixture was heated at 80 °C for 30 min; the measured parameters was found in accordance with the predicted values. High Performance Liquid Chromatography (HPLC) analysis at optimized condition reveals 6 phenolic compounds. The results suggest that optimization of the extraction conditions is critical for accurate quantification of phenolics and antioxidants in Berberis asiatica fruits, which may further be utilized for industrial extraction procedure.
Palabras clave
3-Hydroxybenzoic acid (PubChem CID: 7420); Antioxidants; Ascorbic acid (PubChem CID: 54670067); Caffeic acid (PubChem CID: 689043); Catechin (PubChem CID: 9064); Chlorogenic acid (PubChem CID: 1794427); Extraction optimization; Gallic acid (PubChem CID: 370); Himalaya; Methanol (PubChem CID: 887); Polyphenols; RSM; Rutin (PubChem CID: 5280805); Vanillic acid (PubChem CID: 8468); p-Coumaric acid (PubChem CID: 637542)
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Fenoles
/
Extractos Vegetales
/
Berberis
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Polifenoles
/
Frutas
/
Antioxidantes
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Food Chem
Año:
2016
Tipo del documento:
Article
País de afiliación:
India