Light-induced nuclear export reveals rapid dynamics of epigenetic modifications.
Nat Chem Biol
; 12(6): 399-401, 2016 06.
Article
en En
| MEDLINE
| ID: mdl-27089030
ABSTRACT
We engineered a photoactivatable system for rapidly and reversibly exporting proteins from the nucleus by embedding a nuclear export signal in the LOV2 domain from phototropin 1. Fusing the chromatin modifier Bre1 to the photoswitch, we achieved light-dependent control of histone H2B monoubiquitylation in yeast, revealing fast turnover of the ubiquitin mark. Moreover, this inducible system allowed us to dynamically monitor the status of epigenetic modifications dependent on H2B ubiquitylation.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Histonas
/
Núcleo Celular
/
Epigénesis Genética
/
Ubiquitinación
/
Flavoproteínas
/
Luz
Idioma:
En
Revista:
Nat Chem Biol
Asunto de la revista:
BIOLOGIA
/
QUIMICA
Año:
2016
Tipo del documento:
Article
País de afiliación:
Estados Unidos