Light-induced nuclear export reveals rapid dynamics of epigenetic modifications.

Yumerefendi, Hayretin; Lerner, Andrew Michael; Zimmerman, Seth Parker; Hahn, Klaus; Bear, James E; Strahl, Brian D; Kuhlman, Brian

Yumerefendi, Hayretin; Lerner, Andrew Michael; Zimmerman, Seth Parker; Hahn, Klaus; Bear, James E; Strahl, Brian D; Kuhlman, Brian.

Afiliación

Yumerefendi H; Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, North Carolina, USA.
Lerner AM; Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, North Carolina, USA.
Zimmerman SP; Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, North Carolina, USA.
Hahn K; Department of Pharmacology, University of North Carolina, Chapel Hill, North Carolina, USA.
Bear JE; Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina, USA.
Strahl BD; Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina, USA.
Kuhlman B; Department of Cell Biology &Physiology, University of North Carolina, Chapel Hill, North Carolina, USA.

Nat Chem Biol ; 12(6): 399-401, 2016 06.

Article en En | MEDLINE | ID: mdl-27089030

ABSTRACT

ABSTRACT

We engineered a photoactivatable system for rapidly and reversibly exporting proteins from the nucleus by embedding a nuclear export signal in the LOV2 domain from phototropin 1. Fusing the chromatin modifier Bre1 to the photoswitch, we achieved light-dependent control of histone H2B monoubiquitylation in yeast, revealing fast turnover of the ubiquitin mark. Moreover, this inducible system allowed us to dynamically monitor the status of epigenetic modifications dependent on H2B ubiquitylation.

Asunto(s)

Núcleo Celular/metabolismo; Núcleo Celular/efectos de la radiación; Epigénesis Genética/efectos de la radiación; Flavoproteínas/metabolismo; Histonas/metabolismo; Luz; Ubiquitinación/efectos de la radiación; Flavoproteínas/química; Flavoproteínas/genética; Señales de Exportación Nuclear; Fototropinas/química; Dominios Proteicos; Transporte de Proteínas/efectos de la radiación; Saccharomyces cerevisiae/citología; Saccharomyces cerevisiae/metabolismo; Saccharomyces cerevisiae/efectos de la radiación; Proteínas de Saccharomyces cerevisiae/metabolismo; Ubiquitina/metabolismo

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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Histonas / Núcleo Celular / Epigénesis Genética / Ubiquitinación / Flavoproteínas / Luz Idioma: En Revista: Nat Chem Biol Asunto de la revista: BIOLOGIA / QUIMICA Año: 2016 Tipo del documento: Article País de afiliación: Estados Unidos

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